African trypanosomiasis is a devastating disease to both animals and humans in Sub-saharan Africa. To successfully control the disease, much understanding of the biology of trypanosomes and their vectors is imperative for scientists to establish control strategies. Among many objectives, researchers need to study survival mechanisms of parasites in the host, screen compounds for treatment, study parasite evasion mechanisms of host immunity and chemotherapeutics resistance. Nowadays, in vivo studies have become expensive coupled with animal welfare ethics. Therefore, in vitro studies gain importance to enabling scientists to conveniently conduct their researches. This book explicitly described how to establish and grow the parasites in the cells culture and furthermore gives information on the important elements and parameters one should consider to successfully propagate the parasites outside the animal host. In this book, you will also learn the challenges that can be faced when growing parasites outside their hosts.