Mushroom is considered as the novel complete food because it contains many nutrition’s, vitamins and minerals. Mushroom are a rich source of low calories and are also the, source of fibres, antioxidants and proteins. Mushroom also have the anticancer and anti-inflammatory properties in it. More then 1000+ varieties of mushroom is cultivated worldwide. Mushroom is the only food component which have vitamin D present in it. It is also seen that mushroom can help in treatment of many diseases such as Alzheimer’s, heart disease and cancer which do not have even a proper medical treatment right now available in the medical world also. The handbook complies all the procedure, condition and test related to mushroom cultivation and to check its Phytochemical properties, that will be helpful for researches and common people to devise the working plan for mushroom cultivation. Along with this it also contain the experiment which is done in a lab under sterilization condition that which substrate is best for the mushroom cultivation, and in which substrate the growth of mushroom is early and efficient.