Today, hearing impairment caused by genetic or environmental factors may occur. Among the factors that could cause the hearing loss, genetic mutations that occur in maternal mitochondria. One of the candidate genes for deafness syndrome, a genetic mutation that GJB2 mutation creates 35del G's. In this book, we analyzed the gene. Our findings in this study were similar to other researchers in different countries of the world.Research conducted in different parts of the country reflects the importance of the 35delG mutation in the GJB2 gene. But the mutations listed in the incidence of non-syndromic deafness with autosomal recessive inheritance pattern (ARNSHL) in the city of Tabriz is unknown. In this study, the mutation frequency in the population are non-syndromic hearing was conducted in Tabriz. Non-syndromic recessive deafness Tabrizi 79 non-related patients were studied. Screening of GJB2 35delG mutation that causes some cases of this type of hearing loss is considered, using molecular techniques ARMS - PCR (Allel Refraction Mutation System - Polymerase Chain Reaction) and the nucleotide sequences were determined.