Today, diseases of epidemic disease is a blood disorder worldwide. The anemia of thalassemia disorders have always been a problem for humans. The books on the cause of thalassemia patients is genetically writing in this book from the perspective of genetics, hematology research has been conducted to determine the exact causes thalassemia patients.Thalassemia is the most common cause of anemia, such as alpha and beta globin genes that are involved in different chains and reduces the globin chain synthesis. On average, 5% of the world population, but only one variant globin gene, 1 to 7 percent, alpha and beta thalassemia minor show. Identification of common mutations in beta-thalassemia prevention programs and treatment can help carriers with specific genotypes. The project studied 117 people with thalassemia minor. RFLP technique for the detection of mutations and deletion mutations to detect displacement of ARMS-PCR technique was used and evaluated by SPSS data obtained were Bioinformatics. Among the known mutations, mutations CD36 / 37 (-T) with 59 and one with the mutation IVSII-1 (G> A) mutation with 34 and one with IVSI-110 (G> A) with 24 person maximum their frequency.