In this study, 208 persons without clinical symptoms of heart disease - cardiovascular and venous thrombosis history of Tabriz were evaluated by different tribes. To assess the distribution of the polymorphism of factor 5 Leiden, a genetic heart disease - cardiovascular (CVD), in the city of Tabriz reverse hybridization method was used for the rapid and accurate diagnosis. Under the Multiplex PCR and hybridization experiments on the test strip. The oligonucleotide probes containing allele-specific tape parallel lines. Leiden mutation rate of 5 (%1/2) in the study population than previously published figures in Tehran (% 2/7), respectively. In this study, the distribution of mutant alleles of the 5 Leiden tribal population in the city of Tabriz. The results are compared with other studies. This study is the most comprehensive study to date on the 5-Leiden gene polymorphism was done in the city.