Optical isomerism is one of the topic in chemistry. we had proven that concept using special type of matrix con- s- k-Ep matrix.it was first attempted to be proven and had been widely accepted.Using graph theory and matrix theory it had been proven. the chemical structure of the molecule can be optically isomerised using the grath theory and matrix theory.It can be found that the adjacency matrices and the con-s-k hermition form of these matrices give rise to the same characteristic equation so the characteristic values are also same. Also the con-s-k hermition form of the adjacency matrices gives the isomers of the given molecular structure. Adjacency matrices are also con-s-k-EP matrices.