Determination of Caffeine

Caffeine may be present in organic compounds of the methylxanthine family found within the leaves, seeds, or fruits, of over 60 plant species worldwide. It is used in several soft drinks as a flavoring agent and is deliberately added to make people addicted to these drinks. Caffeine is a natural central nervous system stimulant, having the effects of reducing sleepiness and recovering alertness. Caffeine can produce a mild form of drug dependence associated with withdrawal symptoms such as sleepiness, headache, and irritability when an individual stops using caffeine after repeated daily intake. The spectroscopy technique is used to estimate the caffeine from coffee.